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Ch. 18 - BiotechnologyWorksheetSee all chapters
All Chapters
Ch. 1 - Introduction to Microbiology
Ch. 2 - Disproving Spontaneous Generation
Ch. 3 - Chemical Principles of Microbiology
Ch. 4 - Water
Ch. 5 - Molecules of Microbiology
Ch. 6 - Cell Membrane & Transport
Ch. 7 - Prokaryotic Cell Structures & Functions
Ch. 8 - Eukaryotic Cell Structures & Functions
Ch. 9 - Microscopes
Ch. 10 - Dynamics of Microbial Growth
Ch. 11 - Controlling Microbial Growth
Ch. 12 - Microbial Metabolism
Ch. 13 - Photosynthesis
Ch. 15 - DNA Replication
Ch. 16 - Central Dogma & Gene Regulation
Ch. 17 - Microbial Genetics
Ch. 18 - Biotechnology
Ch. 21 - Viruses, Viroids, & Prions
Ch. 22 - Innate Immunity
Ch. 23 - Adaptive Immunity
Ch. 24 - Principles of Disease
Introduction to DNA-Based Technology
Introduction to DNA Cloning
Steps to DNA Cloning
Introduction to Polymerase Chain Reaction
The Steps of PCR
Gel Electrophoresis
Southern Blotting
DNA Fingerprinting
Introduction to DNA Sequencing
Dideoxy Sequencing

Concept #1: Steps to DNA Cloning

Concept #2: Steps to DNA Cloning

Practice: Human DNA cut with restriction enzyme A can be joined to:

Concept #3: Steps to DNA Cloning

Practice: What is the enzymatic function of restriction enzymes?

Practice: Each restriction enzyme has a specific sequence of nucleotides where it cuts the DNA. These sequences of DNA are unique to each restriction enzyme and are known as:

Concept #4: Steps to DNA Cloning

Practice: The single-stranded ends of DNA molecules can be joined together by:

Concept #5: Steps to DNA Cloning

Practice: The process of using DNA from one organism to alter the characteristics of another is called:

Practice: An organism which has foreign genes incorporated into its genomes is known as a:

Concept #6: Steps to DNA Cloning

Practice: What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I. Transform bacteria with a recombinant DNA molecule.
II. Cut the plasmid DNA using restriction enzymes (endonucleases).
III. Extract plasmid DNA from bacterial cells.
IV. Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments. V. Use ligase to seal plasmid DNA to non-plasmid DNA.