18. Molecular Genetic Tools
Methods for Analyzing DNA
18. Molecular Genetic Tools
Methods for Analyzing DNA - Video Tutorials & Practice Problems
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Methods for Analyzing DNA and RNA
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Problem
ProblemIn terms of molecular biology, what is a library?
A
A place where molecular biology textbooks are stored
B
A collection of clones containing short genetic sequences
C
A collection of clones containing a large collection of proteins
D
A collection of clones with different mutant phenotypes
3
Problem
ProblemIf you had a sample of RNA to analyze, which of the following techniques would you most likely use?
A
Southern Blots
B
SDS-PAGE Blots
C
Northern Blots
D
Western Blots
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PRACTICE PROBLEMS AND ACTIVITIES (78)
- What is CODIS? Describe the four most important features of genetic markers used in this system.
- In this chapter, we focused on a number of interesting applications of genetic engineering, genomics, and biot...
- In this chapter, we focused on a number of interesting applications of genetic engineering, genomics, and biot...
- Compare and contrast the terms Paternity Index (PI) and Combined Paternity Index (CPI). How does each contribu...
- What is the exclusion principle? How is it used in forensic genetic analysis and in paternity determination?
- What is the statistical principle underlying genetic health risk assessment? Why are these assessments not pre...
- You have constructed four different libraries: a genomic library made from DNA isolated from human brain tissu...
- You have constructed four different libraries: a genomic library made from DNA isolated from human brain tissu...
- Using the genomic libraries in Problem 4, you wish to clone the human gene encoding myostatin, which is expres...
- Explain the meaning of 'identity by descent' in the context of identifying genealogical relationship between i...
- Using the genomic libraries in Problem 4, you wish to clone the human gene encoding myostatin, which is expres...
- Using the genomic libraries in Problem 4, you wish to clone the human gene encoding myostatin, which is expres...
- Using the genomic libraries in Problem 4, you wish to clone the human gene encoding myostatin, which is expres...
- A victim of murder is found to have scrapings containing skin cells under several of her fingernails. Genetic ...
- A victim of murder is found to have scrapings containing skin cells under several of her fingernails. Genetic ...
- The results shown are from a DNA test for four genes used in a paternity identification case. DNA for the moth...
- The results shown are from a DNA test for four genes used in a paternity identification case. DNA for the moth...
- The results shown are from a DNA test for four genes used in a paternity identification case. DNA for the moth...
- As genetic testing becomes widespread, medical records will contain the results of such testing. Who should ha...
- Figure E.1 illustrates the results of an electrophoretic analysis of 13 CODIS STR markers on a DNA sample and ...
- Might it make sense someday to sequence every newborn's genome at the time of birth? What are the potential ad...
- Additional STR allele frequency information can be added to improve the analysis in Problem 8. The frequency o...
- Which of the examples of genetic testing below are prognostic tests? Which are diagnostic?DNA sequencing of a ...
- Which of the examples of genetic testing below are prognostic tests? Which are diagnostic?ASO testing determin...
- Which of the examples of genetic testing below are prognostic tests? Which are diagnostic?Individual sequencin...
- Which of the examples of genetic testing below are prognostic tests? Which are diagnostic?An adult diagnosed w...
- Which of the examples of genetic testing below are prognostic tests? Which are diagnostic?Genetic testing in a...
- The frequencies of the four alleles contributed to the child by possible father F1 in Problem 7 are 0.18, 0.23...
- The frequencies of the four alleles contributed to the child by possible father F1 in Problem 7 are 0.18, 0.23...
- Does genetic analysis by ASO testing allow for detection of epigenetic changes that may contribute to a geneti...
- In an inheritance case, a man has died leaving his estate to be divided equally between 'his wife and his offs...
- In an inheritance case, a man has died leaving his estate to be divided equally between 'his wife and his offs...
- Maternal blood tests for three pregnant women revealed they would be having boys, yet subsequent ultrasound im...
- Three independently assorting STR markers (A, B, and C) are used to assess the paternity of a colt recently bo...
- Three independently assorting STR markers (A, B, and C) are used to assess the paternity of a colt recently bo...
- What is the main purpose of genome-wide association studies (GWAS)? How can information from GWAS be used to i...
- Describe how the team from the J. Craig Venter Institute created a synthetic genome. How did the team demonstr...
- In a control experiment, a plasmid containing a HindIII recognition sequence within a kanamycin resistance gen...
- What advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications where the ...
- The bacteriophage lambda genome can exist in either a linear form (see Figures 15.1 and 15.8) or a circular fo...
- To create a cDNA library, cDNA can be inserted into vectors and cloned. In the analysis of cDNA clones, it is ...
- A hereditary disease is inherited as an autosomal recessive trait1. The wild-type allele of the disease gene p...
- A hereditary disease is inherited as an autosomal recessive trait1. The wild-type allele of the disease gene p...
- A 2-kb fragment of E. coli DNA contains the complete sequence of a gene for which transcription is terminated ...
- A 2-kb fragment of E. coli DNA contains the complete sequence of a gene for which transcription is terminated ...
- Variable number tandem repeats (VNTRs) are repeating DNA sequences of about 15–100 bp in length, found both wi...
- Although the capture and trading of great apes has been banned in 112 countries since 1973, it is estimated th...
- The National Institutes of Health created the Genetic Testing Registry (GTR) to increase transparency by publi...
- Should the FDA regulate direct-to-consumer genetic tests, or should these tests be available as a 'buyer bewar...
- Would you have your genome sequenced, if the price was affordable? Why or why not? If you answered yes, would ...
- You have isolated another cDNA clone of the CRABS CLAW gene from a cDNA library constructed using the vector s...
- You have isolated another cDNA clone of the CRABS CLAW gene from a cDNA library constructed using the vector s...
- You have isolated another cDNA clone of the CRABS CLAW gene from a cDNA library constructed using the vector s...
- Following the tragic shooting of 20 children at a school in Newtown, Connecticut, in 2012, Connecticut's state...
- Private companies are offering personal DNA sequencing along with interpretation. What services do they offer?...
- In Drosophila, loss-of-function Ultrabithorax mutations result in the posterior thoracic segments differentiat...
- Genomic DNA from the nematode worm Caenorhabditis elegans is organized by nucleosomes in the manner typical of...
- Genomic DNA from the nematode worm Caenorhabditis elegans is organized by nucleosomes in the manner typical of...
- A family consisting of a mother (I-1), a father (I-2), and three children (II-1, II-2, and II-3) are genotyped...
- You have identified an enhancer trap line (see Figure 14.17) generated by P element transposition in Drosophil...
- You have identified an enhancer trap line (see Figure 14.17) generated by P element transposition in Drosophil...
- A family consisting of a mother (I-1), a father (I-2), and three children (II-1, II-2, and II-3) are genotyped...
- A family consisting of a mother (I-1), a father (I-2), and three children (II-1, II-2, and II-3) are genotyped...
- Suppose you have a 1-kb segment of cloned DNA that is suspected to contain a eukaryotic promoter including a T...
- In a dideoxy DNA sequencing experiment, four separate reactions are carried out to provide the replicated mate...
- In a dideoxy DNA sequencing experiment, four separate reactions are carried out to provide the replicated mate...
- In a dideoxy DNA sequencing experiment, four separate reactions are carried out to provide the replicated mate...
- A three-gene system of additive genes (A, B, and C) controls plant height. Each gene has two alleles (A and a,...
- A three-gene system of additive genes (A, B, and C) controls plant height. Each gene has two alleles (A and a,...
- The following dideoxy DNA sequencing gel is produced in a laboratory. What is the double-stranded DNA sequenc...
- Using an illustration style and labeling similar to that in Problem 29, draw the electrophoresis gel containin...
- A total of 20 men and 20 women volunteer to participate in a statistics project. The height and weight of each...
- A PCR reaction begins with one double-stranded segment of DNA. How many double-stranded copies of DNA are pres...
- Alkaptonuria is a human autosomal recessive disorder caused by mutation of the HAO gene that encodes the enzym...
- The electrophoresis gel shown in part (a) is from a DNase I footprint analysis of an operon transcription cont...
- The electrophoresis gel shown in part (a) is from a DNase I footprint analysis of an operon transcription cont...
- The two gels illustrated contain dideoxynucleotide DNA-sequencing information for a wild-type segment and muta...
- The two gels illustrated contain dideoxynucleotide DNA-sequencing information for a wild-type segment and muta...