Southern Blotting

Practice: A southern blotting technique is used to detect a specific ______ sequence from a blood or tissue sample.

Practice: A _____ is a single-stranded DNA molecule used in hybridization reactions to detect the presence of a particular gene in an assortment of DNA fragments.
(Hybridization Reactions: reactions that combine two complementary single-stranded DNA molecules)

Practice: A geneticist wants to see if her patient has Gene X. The geneticist takes a blood sample from her patient and prepares a southern blot. How will the geneticist know if her patient possesses Gene X?

Practice: Place the following steps of Southern Blotting in the correct order.
a) ______: Filter paper is incubated with the labeled DNA probe which anneals to the ssDNA fragments.
b) ______: Analyze gel to determine the presence of the DNA sequence of interest.
c) ______: Separate DNA fragments by size using gel electrophoresis.
d) ______: Denature DNA by soaking gel in a basic solution.
e) ______: Fragment unknown DNA sample(s) using restriction enzymes.

Practice: By analyzing the Southern Blot results below, which of the samples contains the gene of interest?