Km Enzyme

Practice: What is the initial velocity of a reaction when the concentration of substrate is set equal to the K_m?

Practice: Select the best description of the K _m.

Practice: According to the chart below, which one of the following enzymes has the strongest affinity for its substrate?

Practice: Indicate which region of the Enzyme Kinetics plot below best corresponds to each statement.

A) Initial reaction velocity is limited mainly by the [S] present: ______

B) Initial reaction velocity limited mainly by the [E] present: ______

C) The active site of an enzyme is most likely free/unoccupied: ______

D) The active site of an enzyme is most likely occupied by substrate: ______

E) This region includes the points corresponding to Km & ½Vmax: ______

Practice: Use the data in the following chart to determine the Km of the enzyme.