Practice: Use the data in the chart below to determine the answer to the following:
A) Rank the enzymes in order of their binding affinity to their substrate (strongest affinity → weakest affinity).
a) A → B → C.
b) C → A → B.
c) C → B → A.
d) B → A → C.
B) Rank the enzymes in order of their binding affinity to the inhibitor (strongest affinity → weakest affinity).
a) A → B → C.
b) B → C → A.
c) C → B → A.
d) B → A → C.
C) Which enzyme would you expect the inhibitor to affect the most? Why?
a) Enzyme A.
b) Enzyme B.
c) Enzyme C.